Flow cytometry has become a crucial part of modern biochemistry, immunology and regenerative medicine research. Routine analysis using flow cytometry, is forming the basis of large numbers of experiments and flow cytometry technologies allow the capture of large data sets. Due to the wide variety of users from Monash University, CSIRO and Industry, the storage, analysis, handling and accessibility of such data has posed significant challenges to the research community. At present the responsibility of requesting and maintaining data falls to individual researchers and/or research groups. We propose that a centrally managed and integrated data collection with common data handling solutions is required to (i) facilitate discovery and analysis of the data (ii) facilitate sharing of data with collaborators and after review with the wider research community, (iii) mitigate the risk of data loss and (iv) provide a framework for enhancing data accessibility and development of future data analysis platforms. This Collection will make cytometry data from research grants totalling $59M accessible to researchers and provide a mechanism to make published data available for re-use.