Microscopy imaging has become a crucial part of modern genetic, cell and developmental biology research. In recent years, new microscopy techniques and technologies have lead to a dramatic increase in the size and dimensionality of image data. As a result, the storage, analysis, handling and accessibility of such data has posed significant challenges to the research community. At present, the responsibility of requesting and maintaining image data falls to individual researchers and/or research groups. We propose that a centrally managed and integrated image data collection with common data handling solutions is required to (i) facilitate discovery and analysis of the data, (ii) facilitate sharing of imaging data with collaborators and after peer5review with wider research community, (iii) mitigate the risk of data loss and (iv) provide a framework for enhancing data accessibility and development of future image analysis platforms. This Collection will make imaging data from research grants totalling $25M accessible to researchers and provide a mechanism to make published data available for reFuse.