Next Generation Sequencing of Extracellular RNA

Exosomes are small nanovesicles released by cells. They can be found in bodily fluids such as blood, milk, saliva and cerebrospinal fluid, and be isolated from cell culture supernatants. Exosomes contain protein and genetic material and have been proposed to play a role in cellular communication whereby their contents can be transferred to other cells. We have shown a role for these vesicles in a number of neurodegenerative disorders such as prion, Alzheimer’s and Parkinson’s diseases. In addition, we have developed a next generation sequencing (NGS) platform to sequence the genetic material contained within these vesicles. The collection contains data from our studies sequencing exosomal RNA from a variety of sources including cell cultures, serum, plasma, milk, urine, CSF both to look at the normal content of these vesicles and also to identify potential biomarkers for the diseases we are studying.